Cryo-ultramicrotomy Minicourse

Date: October 17-18, 2016

Location: University of Maryland Baltimore, Electron Microscopy Core Imaging Facility (EMCIF)

Instructor: Dr. Helmut Gnaegi, Diatome Ltd

Lecturer and demonstrator: EMCIF Director (Dr. Ru-ching Hsia)  and staff

Scope of aims:  To promote the knowledge and skills of advanced cryo-ultramicrotomy techniques

Targeted participants:  Individuals who are experienced with ultramicrotome operation at ambient temperature and wish to extend their skills to include sectioning CEMOVIS and Tokuyasu immunolabeling specimen

Format: Lecture, demonstration and hands-on practice

Techniques covered: High pressure freezing of  samples for Cryo electron microscopy of vitreous sections (CEMOVIS), Cryo-sectioning of CEMOVIS samples, Cryo sectioning of sucrose infused biological sample (for Tokuyasu immunolabeling)

Instrumentation: High pressure freezer ( Leica EMPACT II); Cryoultramicrotome (Leica UC6/FC7); Two Micromanipulator (EMS/Diatome)

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Companion IGSS workshop on October 19 to 21 will cover Tokuyasu immunolabeling techniques along with post-embedding and pre-embedding immunogold labeling techniques.  Participants of cryo ultramicrotomy course that are interested to  learn the whole Tokuyasu immunolabeling methods are strongly encourage to attend IGSS workshop as well. 

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Course agenda  will be available soon

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Registration Fee: $600   ($550 if also attend Aurion IGSS workshop on 10/19-10/21, 2016)                          

Participants: maximum 6

Register Now

Regisstration will close when all spaces are filled.

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Payment methods:

An invoice will be e-mailed to you within five business days upon the receipt of registration.

Registration can be paid by check or credit card.

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Lodging: A list of nearby hotels that offer special rate for UMB guests is available upon request, please contact EMCIF.

Other inquiry: E-mail coreimaging@umaryland.edu