Cryo-ultramicrotomy Minicourse
Date: July 27-28, 2015
Location: University of Maryland Baltimore, Electron Microscopy Core Imaging Facility (EMCIF)
Instructor: Dr. Helmut Gnaegi, Diatome Ltd
Lecturer and demonstrator: EMCIF Director (Dr. Ru-ching Hsia) and staff
Scope of aims: To promote the knowledge and skills of advanced cryo-ultramicrotomy techniques
Targeted participants: Individuals who are experienced with ultramicrotome operation at ambient temperature and wish to extend their skills to include sectioning CEMOVIS and Tokuyasu immmunolabeling specimen
Format: Lecture, demonstration and hands-on practice
Techniques covered: High pressure freezing of samples for Cryo electron microscopy of vitreous sections (CEMOVIS), Cryo-sectioning of CEMOVIS samples, Cryo sectioning of sucrose infused biological sample (for Tokuyasu immunolabeling)
Instrumentation: High pressure freezer ( Leica EMPACT II); Cryoultramicrotome (Leica UC6/FC7); Two Micromanipulator (EMS/Diatome)
2015 Cryoultramicrotomy Course Program
Registration Fee: $600
Participants: maximum 6
Inquiry: E-mail coreimaging@umaryland.edu